6. Studies on Nucleic Acid and Related Substances

Antagonists of nucleic acid derivatives. II. Reversal studies with substances structurally related to thymine.

One of the major problems of antimetabolite studies is the evaluation of the rale of “secondary reversing agents” (1). In a system in which there exists a competitive relationship between a metabolite and its antimetabolite, the effect of a secondary reversing agent is to increase the ratio of antimetabolite to metabolite required to produce a given inhibition; the secondary reversing agent can...

Reactions of 6-aminopenicillanic acid with carbohydrates and related substances.

Studies on Nucleic Acid Metachromasy

Acrolein-fixed, polyester wax-embedded tissue sections showed excellent preservation of light microscopic architecture and, when stained with toluidine blue, intense color contrast between DNA, which stained orthochromatically, and RNA, which stained metachromatically. This method has practical value for differentiating DNA from RNA in the same section. The color contrast was impaired by substi...

NMR studies on protein-nucleic acid interaction.

A special section of this issue is devoted to the study of protein-nucleic acid interaction by NMR. This topic constitutes a fruitful area of research already for several decades. Interactions between proteins and nucleic acids are involved in many essential cellular processes such as replication, transcription, translation, DNA repair, and the regulation thereof. Structural and dynamics studie...

Identification of Low-Molecular-Weight Nucleic Acid-Related Substances Secreted by Streptomyces aureofaciens.

Streptomyces aureofaciens growth in chemically defined medium is actively associated with the secretion of low-molecular-weight nucleic acid-related substances and is linked to low availability of phosphate. Thirteen pure compounds were isolated, of which seven were identified.